The cookies is used to store the user consent for the cookies in the category "Necessary". This cookie is set by GDPR Cookie Consent plugin. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. Principles of Inheritance and Variation.New Questions and Answers and Forum Categories This causes slow ripening of tomatoes, thus improving their shelf life. These inhibite the expression of gene and produce less PG enzyme. Transgenic tomatoes have been developed which contain antisense strands of poly-galactouronase gene. Application of antisense technology in plant genetic engineering. Aegis Therapeutics, a drug company have developed an antisense drug that can be administered orally.ġ. It has been discovered that antisense RNA can target Mycobacterium tuberculosis genes. Scientists recently have developed a new class of drugs called antisense drugs which can block the genes like oncogenes, associated with the production of harmful proteins.Īntisense therapy is showing promise against one of the greatest killers, tuberculosis. In human beings, mRNAs transcribed from the viral genome or from the mutant oncogenes can be destroyed using RNA interfering technology, while at the same time not affecting any other mRNAs of the cell.Īntisense technology offers a technique for inactivating a single chosen gene. This is a powerful technology tool which can selectively silence any gene. Proteins encoded by other mRNAs are not affected. In this way it inhibits the translation of mRNA or they can move into the nucleus where they cause chromatin modification. Taken up by an enzyme called RNA induced silencing complex (RISC) to destroy the corresponding mRNA. One strand of SiRNA is destroyed and its antisense strand is These are known as small interfering RNAs (Si RNAs). Inside the cell the double stranded RNA is first cleaved by a ribonuclease called Dicer, into small double stranded fragments of 21-23 nucleotides. They inhibit the expression of similar genes in plant cell. Many plant viruses have double stranded RNAs with sequences similar to genes of the plant cell. This leads to selective inhibition of gene expression. RNA Interference (RNAi):ĭouble stranded RNAs (ds RNAs) play a major role in selective destruction of mRNAs which have the same sequence as the ds RNAs. It has also been discovered that antisense RNA can also prevent transcription of genes.Īntisense RNA is produced by reversing the orientation of a gene with regards to its promoter so that antisense strand is transcribed. This offers a powerful technology tool for turning off genes or silencing genes at will. This is known as post transcriptional gene silencing. In this way translation of mRNA is prevented. ![]() The translation of mRNA is inhibited by binding to antisense RNA which is complementary to mRNA. It may bind to RNA to form RNA-RNA duplex structure. An antisense RNA is an RNA strand that is complementary to the mRNA strand.
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